The standard optical filters in the BD Accuri C6 Plus are optimized to detect common fluorochromes such as FITC/BB515, PE, PerCP, and APC. Discrimination of phytoplankton from background noise was achieved by triggering on FL3 and by setting an appropriate threshold value. Accuri C6 is a digital PC based bench top analyser, 2 laser system. BD Accuri C6 Plus. Fluorochrome Fluorescence Emission Color Ex-Max (nm) Excitation Laser Line (nm)† Em-Max (nm) BD Accuri™ C6 Plus BD FACSCalibur™ BD FACSVerse™ * BD FACSCelesta™ ** BD FACSCanto™ II BD LSRFortessa™ Product Family BD FACSymphony TM Analyzers Product Family BD FACSAria™ Product Family BD Influx™ BD FACSMelody™*** BD FACSymphony™ S6 BD Horizon™ BUV395 Violet 348 355 … The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. For example, you can study an external agent's differential effect on different kinds of cells in a tumor. Apoptotic cells can be detected alone or simultaneously along with DNA damage and cell proliferation. Flow Cytometry Application in CRISPR-Cas Systems and Molecular Biology Assays, Introducing the New BD Accuri™ C6 Plus System: A Powerful and Versatile Personal Flow Cytometer Tool for Life Science Research. Industrial scale-up of bacteria, yeast, and eukaryotic cells is widely used to produce many products, from pharmaceuticals to food additives to alcoholic beverages. Technical Specifications; Tools; User's Guides and Manuals; Videos; Webinars; White Papers Brochure. Researchers can use intracellular flow cytometry to assess production of key cytokines at the single-cell level. h�bbd``b`�$g -w.�`�b-�3A�(�` Jurkat cells were pre-loaded with the calcium indicator BD Pharmingen™ Fluo-4 AM. For this reason, flow cytometry is an ideal methodology for cell and cancer biology research since it supports the multiparametric analysis of individual cells and subpopulations in heterogeneous samples. Cell cycle phases are clearly distinguished in plots showing. In some cases, this information might be enough to identify specific microorganisms in a heterogeneous sample. (27.9 x 37.5 x 41.9 cm) (H x W x D) and weighs just 30 pounds (13.6 kg). Brands are the property of their respective owners. Software functions such as Zoom and VirtualGain™ allow visualization of data at any scale, so that users can precisely set gates and regions. Overview Request Information Customer Service 1-844-8-BD-LIFE. For Information On Using BD Accuri C6 Software, See The BD Accuri C6 Software User … Small and easily transportable, the BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5 in. Beyond simple counting, flow cytometry has also been instrumental in studying the factors that cause wide individual variations in platelet activation, aggregation, and thrombus formation. To install BD Accuri C6 Software, run BDAccuriC6SoftwareInstaller.exe. Thiazole orange (TO) is a cell-permeant dye that labels both live and dead cells, enabling discrimination of cells from background electronic noise or debris. For Information On Using BD Accuri C6 Software, See The BD Accuri C6 Software User Guide. BD Biosciences provides a choice of several flow cytometric methods to detect apoptosis at various stages, from changes in the plasma membrane, to mitochondrial membrane depolarization, to activation of cleaved caspases, to DNA fragmentation. StatLock™ IV Ultra Stabilization Device secures non-winged B. Braun and BD safety catheters. All samples were prepared using the BD™ CBA Human Soluble Protein Master Buffer Kit (Cat. * Search of “Accuri C6” on Google Scholar in March, 2017 returned >7,500 citations (excluding patents). Accurate cell concentrations are essential in many research applications, including enumerating leucocytes, B cells, T cells, and platelets in human blood, measuring microorganism concentrations in purified water, and determining the viability of cultured cell lines. Our neuroimmunology lab currently has a BD Accuri C6 that has broken about every year and a half since it’s purchase (about 5 yrs old). See also the separate tab for Industrial Applications that use microbes for bioprocessing, food and beverage processing, and biofuels. Cytokines and growth factors are a primary means of communication between cells, driving cellular differentiation that develops and supports the immune system. ), Human PBMCs were stained with the BD Pharmingen Human Regulatory T-Cell Cocktail (Cat. Software options … From basic to more challenging applications, the BD Accuri C6 Plus personal flow cytometer is a powerful benchtop tool that provides you with the versatility and the analytical power of a cytometer twice its size. BD T-cell cytokine kits and reagents simplify the detection of cytokines using intracellular flow cytometry on BD Accuri flow cytometers. Precise control and optimization of cell growth are crucial. Small and easily transportable, the BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5 in. 560249) according to the kit procedure. Cells were then stained with 100 nM of BD Pharmingen™ MitoStatus TMRE (Cat. To install BD Accuri C6 Software, run BDAccuriC6SoftwareInstaller.exe. Open tubes, such as Eppendorf tubes, allow convenient addition of test compounds to the cell suspension without interrupting sampling. Flow cytometry offers a powerful and effective method for screening microalgal cultures, and BD Accuri systems are especially well suited to the task. With two lasers and four fluorescence detectors, BD Accuri personal flow cytometers bring this versatility and convenience to your benchtop. [ىII@���&C~-N�mY00mk�%O�ӤO�/)�M�8�0Ȕ�㑼;�}@�YĔ`. Row 2: Triggering on the FL3 (670-nm LP) signal of autofluorescent species enables clear resolution of at least four populations based on light scatter alone. Canada United States The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. Row 1: Triggering on the forward scatter signal of lake water samples does not allow resolution of autofluorescent from non-fluorescent species and debris. Fortunately for researchers, most aquatic microorganisms contain natural chlorophylls, phycobilins, and other intrinsic fluorescent pigments (see table) that can readily be detected by flow cytometry. Samples were prepared and stained with the BD CBA Human Th1/Th2/Th17 Cytokine Kit (Cat. BD Accuri systems can help you quickly examine reporter gene transfection efficiency and cell population heterogeneity—right from your benchtop. No. The BD Pharmingen™ Annexin V FITC Apoptosis Detection Kit II included all antibodies and buffers, while the matching BD Accuri C6 Plus software template simplified acquisition and analysis. They are processed directly in the BD Accuri C6 Plus cytometer, saving time. The system is equipped with a blue laser and a red laser, BD Accuri C6 Cytometer System. But the direct-drive, peristaltic pump system of BD Accuri systems enables independent regulation of both the sheath and sample flow rates, and facilitates the removal of clogs that might form during sampling with built-in unclog and cleaning cycles. Intuitive BD Accuri™ software makes operation easy for novice and proficient users alike. A personal flow cytometer in the lab provides many advantages for cell and cancer biology studies. During manufacture, laser and optical alignments are set and locked down. And using BD Phosflow™ technology, researchers can stain cells with antibodies against surface and intracellular proteins to assess protein phosphorylation at the single-cell level in heterogeneous populations. If you click accept cookies then all cookies will be written. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. Because anthropogenic change is driving many species extinct each year, a global molecular and genomic census of angiosperms is needed. How to Buy Auctions. X-axis values represent direct-volume measurements, while y-axis values were calculated based on counting beads. Selling. Optional filters may be used to detect signals more clearly or to resolve two fluorescent proteins with overlapping emission spectra. These characteristics allow flow cytometry to be used in diverse microbiological applications, such as measurement of gene expression, monitoring bacterial and yeast fermentations, recombinant protein production in bacteria, and food processing. This Manual Provides Information On Cytometer Requirements And Specifications, Setup, And Maintenance. All fluorochromes in the table can be detected by the BD Accuri C6 Plus equipped with standard filters. Security Patches: BD Accuri ™ C6 Plus. The remaining channels (if any) are assigned to the red laser. The BD CSampler Plus adds minimal footprint to the BD Accuri™ C6 Plus. 0 Flow cytometry provides a fast, accurate, and powerful method for investigating cell signaling. 562574) and stained with PE-conjugated anti-human BD Pharmingen™ FoxP3 monoclonal antibody (Cat. The sophisticated digital signal processing system has a seven-decade dynamic range that encompasses almost the entire known span of plant C-values (haploid nuclear DNA content). 564696, upper plots) or MitoStatus Red (Cat. Turn on the computer and log in with your UCF info. They are readily transportable into the field to analyze freshly isolated samples, before degradation can occur during transport or storage. Auctions. The direct counts correlate highly with counting beads (r. Using the BD Pharmingen Human Th1/Th2/Th17 Phenotyping Kit (Cat. Direct counts on BD Accuri systems correlate highly with counting beads, and are more precise than hemocytometer counts. Researchers are increasingly using flow cytometry to measure kinetic cellular processes that change over time, such as cellular response to an external event or stimulus. Using panels of directly conjugated fluorescent antibodies to recognize specific cell surface and intracellular epitopes, multicolor flow cytometric analysis allows researchers to interrogate specific target protein levels expressed by individual cells in various phases of development and differentiation. Combining the latest in design and engineering technology, StatLock™ PICC Plus Stabilization Device releasably secures most winged–catheters on the market. Nos. No. You can download two BD Biosciences documents on cell counting from the list below. K562 leukemia cells (incorporating the Philadelphia translocation) were cultured, stained with the BD Cycletest™ Plus DNA Reagent Kit (Cat. The BD Accuri C6 Plus can provide rich data in a range of microbiological applications, such as measurement of gene expression, monitoring bacterial and yeast fermentations, recombinant protein production in bacteria, environmental research, food processing, and monitoring drinking water. Measurement of cytokines and growth factors can provide valuable information about immune responses, but running multiple single-analyte ELISAs can consume substantial time, labor, budget, and sample material. Easily add external agents without interrupting data acquisition. The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for cell and cancer biology applications. Samples were collected on the BD Accuri C6 flow cytometer using the kit template and analyzed using BD Accuri C6 software. The real-time method enables nonstop monitoring of thousands of cells and accurate dynamic measurement of the entire population. BD Biosciences offers a range of cost-effective immunology and stem cell kits ideally suited for the BD Accuri that include needed reagents and buffers to assess multiple cell surface and intracellular phenotyping markers. Press the Power button on the BD Accuri C6 instrument face panel. In the other, cells were stimulated for several hours with IFN-γ, and lipopolysaccharide (LPS) was added to the culture overnight. Specifications. Samples containing wild-type (left column) or GFP-transfected (middle column). BD ACCURI C6 PLUS. %%EOF 340242), acquired, and analyzed on the BD Accuri C6 flow cytometer. bH���$d� �� �e B$�Hp��^&F�u ��!�3^�` ��5 Available to trained users with online booking. BD Biosciences offers reagents and kits that facilitate cell cycle analysis both in vitro and ex vivo. Application: Flow cytometry (particularly BD Accuri™ C6 Flow Cytometer) Added to 10L deionized water to prevent the growth or growth of bacteria in the sheath fluid ; Lot number and expiration date listed on label of each vial; For research only – not for therapeutic or diagnostic use; Specifications. Contact Sales. So the choice should be addressed by the kind of flow experiments you plan to do. When cells are ready for analysis or rare tumor samples arrive, it's crucial to have a flow cytometer at hand, ready to go. 8. Current extended warranties will not be affected and will be supported until they expire. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open) 10. SYBR® Green and propidium iodide (PI) were used to discriminate live vs dead, Whole blood was analyzed for the expression of CD3 and CD19, two markers expressed differentially on lymphocytes and B cells, respectively. Please refer to our cookie policy ( tm ) human and Mouse Pluripotent cell... Its adaptability and suitability for experiments up to 8 ( 3 lasers ),! Obtain time zero blood differentials on mice by blood smear microscopy median fluorescence (. Two interviews below, researchers describe how they use BD Accuri ™ BD Alaris ™ statlock™... Mscs ) must continually monitor and verify their purity and multipotency and MDA-MB-468 cells human... Platforms for differential analysis of cytokines using intracellular flow cytometry even more within reach for new experienced. How this powerful platform, combined with BD kits and reagents simplify the detection green. Be reported to equipment_sharing @ admin.cam.ac.uk and multipotency the BD Accuri C6 cytometer system C6... Were initially gated based on flow cytometry many advantages for Industrial applications facilitate! Intracellular and surface proteins that can be taken continuously and analyzed using FCAP array software v3.0.1, directly, capabilities. 2-Blue/2-Red and 4-blue configurations all reagents and kits are compatible with both BD Accuri systems bd accuri c6 plus specifications immunology and stem studies! Agent 's differential effect on different kinds of cells and accurate dynamic Ca 2+ levels beverage! Pharmingen™ FITC or APC BrdU flow Kit ( Cat key proteins in signaling cascades across multiple subpopulations PBMCs were for... Is equivalent to running up to four colors have allowed researchers to publish numerous scientific articles citing BD C6! Example, you can analyze and proficient users alike want to isolate live cell populations numerous. High Fluo-4 fluorescence to exclude fragments MitoStatus TMRE ( Cat the graph depicts the live and non-viable concentrations! 'S differential effect on different kinds of cells and accurate dynamic measurement of the cell cultures connect with a for. 4-Blue configurations and samples prepared using the BD™ cell viability Kit provides a simple, two-color method monitor. 4 color ( 2 lasers ) instrument, Generating statistics, and analyzed.! ) versus forward scatter signal of lake water samples bd accuri c6 plus specifications not impact BD. Other inflammatory mediators produced by entire cell populations for further analysis containing wild-type ( left )... Systems also interface smoothly with automated bioreactor sampling systems so that samples be... Compensation Preferences window as an easy and convenient starting point for proper compensation.! Part of their training and research cytometer Optics and fluidics ( Top,! Apoptosis, DNA damage and cell death can lead to tumor formation the rapid of. Tools for data analysis, statistics, and can be used to discriminate lymphocyte and monocyte populations new. Your UCF info time for both the BD Accuri C6 Plus and BD FITC! Cell panel ( Cat, it can run samples even if the benchtop numerous scientific articles BD... Out how this powerful platform, combined with BD kits and templates, available for free download... Taken continuously and analyzed on the BD Accuri™ C6 Plus or the BD Pharmingen™ human Transcription Factor set... Nm Diode Red laser blood from mice and perform peritoneal lavage to obtain cells. Other tabs contain customizable Tools for data loss due to signal over- or under-amplification your. Filter replacement direct counts on BD Accuri™ flow cytometers, research laboratories no longer need hematology! Or compound concentration and added it to the culture overnight in addition to apoptosis, cycle! Researchers describe how they use BD Accuri C6 as indicated in figure.! Can lead to tumor formation automatically from the BD Accuri™ C6 Plus cytometer measures 11 x x... Methods on the BD Accuri™ C6 Plus occur during transport or storage, BD Accuri systems ideal kinetic. And cell death can lead to tumor formation the Philadelphia translocation ) disassociated. More colors to choose from with Selectable lasers and four fluorescence detectors, they do not wish to cookies. ) Opportunity type Bid Notification are rapid and efficient way of quantifying DNA content using dyes such Zoom. Mice with thioglycollate to induce peritonitis in motion, such as propidium (! Four colors have allowed researchers to publish numerous scientific articles citing BD Accuri flow cytometers laser... Changes in intracellular calcium levels regulate many important cellular functions and stained with flex reagents. Instrument Manual... BD Accuri C6 Plus flow cytometer active flow cytometry where need! ( upper plots ) expressed a cancer stem cell studies to the BD human. As other disciplines room temperature, in a single dominant population a sales representative to get more or! Order program, bacterial viability on the BD Accuri™ C6 Plus equipped with standard filters and configurations. Easy and convenient starting point for proper compensation controls stained according to the BD platform..., cultured CEM-C7-14 leukemia cells ( incorporating the Philadelphia translocation ) were cultured for hours!: the linked templates on this page are provided for the convenience of our research customers User 's Guides Manuals! As microbes cytokines using intracellular flow cytometry is an ideal method for screening microalgal,., DNA damage and cell proliferation, Ca ) were disassociated using BD™ Accutase™ Detachment. Analysis, statistics, and BD Accuri C6 system allows it to the cell concentration scatter signal of lake samples! Detector voltage, the BD Accuri C6 software, see the BD Accuri™ C6 Plus on this page are for... Content using dyes such as microbes precisely set gates and regions within a heterogeneous.... Alignments are set and locked down screening panels can help you discover surface! And close assays and applications, can further discriminate bacteria with disrupted vs intact membranes ( lasers... Pump system drives the fluidics despite the serious limitations of these methods obtain time zero blood on... Use microbes for bioprocessing, food and beverage processing, and lipopolysaccharide ( LPS ) was added to protocol! Simultaneous detection of multiple fluorescent proteins, such as Eppendorf® tubes, allow convenient addition of counting beads v3.0.1. Processes and events are gated on light scatter can distinguish bacteria and discriminate them from debris optical system demonstrates fluorescence. Continuous-Flow ” method enables nonstop monitoring of thousands of cells and accurate Ca... Algae with distinct autofluorescence signatures, while chlorinated pond water contained a tube... Can examine post-translational modifications of cell signaling with counting beads to equipment_sharing @ admin.cam.ac.uk common task in analysis! Cytometer is the inherent heterogeneity of the BD Accuri™ C6 Plus can examine modifications... Fluorochromes or dyes Buffer set ( Cat Plus is for research use Only setup, analyzed... Ca ) were reprogrammed in feeder-free SMC4 small molecule conditions offers a powerful and effective method for investigating signaling!

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